In addition, in vitro experiments also showed that ALT-1 (0, 80, 150, 200 μM) reduced the viability and proliferation of COLO205 and HCT116 cell lines, and downregulated the phosphorylation of proteins associated with the AKT/mTOR pathway to inhibit the growth of tumor cells (Wang et al., 2020). This evidence concerns the gene GPT and neoplasm.