Moreover, the therapeutic effect of FAN (0.1 and 0.5 mg/mL) was also demonstrated in an in vivo CRC xenograft model mouse, and effectively suppressed the tumor volume and weight, and this experiment elucidated that FAN blocked the PI3K/AKT signaling pathway in order to exert the anti-tumor activity (Jiang et al., 2021). This evidence concerns the gene AKT1 and neoplasm.