BCL2 and colorectal carcinoma: In addition, in SW480 cell assay, CK-8 and colony formation assay showed that cell proliferation and viability were inhibited, flow cytometric analysis showed that Tan IIA induced apoptosis in CRC cells, and western blot analysis showed that the expression of Bax protein was significantly upregulated in Tan IIA group, and the expression of Bcl-2 protein was significantly reduced.