In addition, Osthole (0, 5, 10, 20, and 50 μM) significantly upregulated the levels of ROS and Beclin1, downregulated the ratio of LC3B-I/LC3B-II, disrupted mitochondrial homeostasis, decreased ATP production, and increased the level of calcium ions in HCT116 and HT29 cells, effectively inhibiting CRC cell viability and proliferation (Song et al., 2024). This evidence concerns the gene BECN1 and colorectal carcinoma.