Finally, high-impact frameshift mutations in TCF7L2 (VAF = 0.133 in HCC cell line and VAF = 0.024 to 0.094 in HCC tumor regions) and CACNA1E (VAF = 0.089 in HCC tumor) were identified in the hepatic fibrosis pathway. This evidence concerns the gene CACNA1E and Hepatic fibrosis.