Use of our new pig exome sequencing kit in this study led to the discovery of 3 novel unique Meishan high-impact variants in the form of premature stop codons (not previously identified in the PigVar database or the EVA) in genes associated with obesity and metabolic syndrome (PLIN1, GRB10, and ACOT4). Here, PLIN1 is linked to obesity due to melanocortin 4 receptor deficiency.