TLR8 and infection: An Mtb mutant that over-produces MVs, Tn:Rv0431 (ΔvirR)46,47 (Figure S6), was able to enhance TLR8 activation (in an RNase-inhibitable manner) during the infection of wild-type (WT), but not TLR8−/−, THP1 macrophages (Figure 2C).