The expression of these genes was screened for prognostic impact using a multivariate Cox proportion hazards model measuring the effects of the mRNA expression of each of the genes and considering the impact of TGFB1/2/3 and MGMT methylation, controlling for age, sex, and age interactions; it yielded 20 genes that showed an OS impact for the gene and MGMT methylation upregulated in tumor tissues (Table S3) in addition to genes representing CD3D (T-cell marker), CD3E (T-cell marker), and CD86 (M1-like macrophage marker) (Figure 4A). Here, TGFB1 is linked to neoplasm.