Recent studies have demonstrated that ΔΔG is a useful tool for evaluating other MVs in genes such as transcription factor FOXD2 and TBC1D31 which are now implicated in syndromic congenital anomalies of the kidney and urinary tract (Riedhammer et al., 2024; Saygılı et al., 2023), as well as NUP85, associated with steroid-resistant nephrotic syndrome (Şükür et al., 2025). This evidence concerns the gene TBC1D31 and nephrotic syndrome.