GSDMD and alkaline phosphatase measurement: Western blot analysis further demonstrated that, relative to the model + TMPZ + NC siRNA group, transfection with NRF2 siRNA attenuated the inhibitory effect of TMPZ on the expression of pyroptosis-related proteins NLRP3, ASC, caspase-1, and GSDMD in AP cells (Figure 5g, P < 0.001).