According to our findings, TMPZ pretreatment enhanced the expression of NRF2 and HO-1, and NRF2 knockdown partially reversed the protective effect of TMPZ pretreatment, confirming that TMPZ exerts its protective role in AP cells by inhibiting cerulein-induced pyroptosis via the NRF2/HO-1 pathway. This evidence concerns the gene HMOX1 and alkaline phosphatase measurement.