Coupling changes in local [O2] with hyperinsulinemic-euglycemic clamp in young healthy animals has demonstrating that fixing local muscle PO2 eliminates the insulin mediated hyperemia, providing evidence of an oxygen dependent component of the response (Wells et al., 2023); however, the impacts of coupling hyperinsulinemic-euglycemic clamp and O2 has yet to be evaluated in a T2D rodent model. The gene discussed is INS; the disease is type 2 diabetes mellitus.