SIRT3 and Cognitive impairment: To investigate the involvement of the AMPK/SIRT signaling pathway in the neuroprotective effects of the ZaTm extract during the progression of scopolamine-induced cognitive deficits, we assessed the phospho-AMPK (p-AMPK)/AMPK ratio and SIRT1 and SIRT3 levels in the hippocampus using western blotting.