To investigate the involvement of the AMPK/SIRT signaling pathway in the neuroprotective effects of the ZaTm extract during the progression of scopolamine-induced cognitive deficits, we assessed the phospho-AMPK (p-AMPK)/AMPK ratio and SIRT1 and SIRT3 levels in the hippocampus using western blotting. This evidence concerns the gene SIRT1 and Cognitive impairment.