Because in addition to alkylating agent treatment chemotherapy treated GBM patients, low MGMT is also associated with longer survival in radiation treated GBM patients and in some studies GBM patients independent of treatment (Hegi et al., 2005) we compared basic neoplasia-associated biological properties of MGMT KO/KD and MGMT wildtype GBM cells. The gene discussed is MGMT; the disease is glioblastoma.