The mitigating effect that RIC has on ROS and oxidative stress has also been demonstrated in animal models of ischaemic stroke, possibly related to upregulation of nuclear factor-E2-related factor 2/heme oxygenase-1 pathway (Nrf2/HO-1) that plays a crucial role in upregulating expression of various antioxidant defence and anti-inflammatory genes [55]. This evidence concerns the gene HMOX1 and ischemic stroke.