To evaluate the functional impact of this coregulated CAR expression, we cocultured EST-109 and CD19 + EST-109 melanoma cells at a 1:1 ratio with either mock, 2xAARE-YB-CAR-T or 2xAARE-YB-CAR-Jun-T cells under AA-restricted conditions (Supplementary Fig. 6D). This evidence concerns the gene CD19 and melanoma.