Given our findings from the mouse studies, we decided to investigate human tumor-reactive CD8 + T cells using publicly available processed and annotated scRNA-seq data from 10 patients with primary colorectal cancer tumor (PT), surrounding colonic tissue (PN), metastatic intrahepatic tumor (MT), surrounding hepatic tissue (MN), tumor-draining lymph nodes (LN), and peripheral blood mononuclear cells (PBMC) (Fig. 3A)24. Here, CD8A is linked to cancer.