Across all macrophage clusters, SPP1 and FN1 ligands from macrophages interacted with CD44, as well as integrin receptors ITGA4_ITGB1 and ITGA4_ITGB7, on pTRT cells to a greater degree in the intrahepatic TILs as compared to primary CRC TILs (Fig. 3M). Here, SPP1 is linked to colorectal carcinoma.