To confirm that the inhibition of tumor growth was due to the expansion of melanoma antigen-specific CD8+T cells after vaccination with the multi-epitope DC vaccines, C57BL/6 mice were immunized with the multi-epitope DC vaccine, DEC-gp100-Trp2, or with one of the single-epitope DC vaccines, DEC-gp100 or DEC-Trp2. Here, CD8A is linked to melanoma.