To investigate the effects of various cytoskeletal inhibitors on brachyury levels, the chordoma cell line U-CH1 was treated for 48 h with CuB, Latrunculin B (LatB), Narciclasine, Colchicine, and Vincristine, adopting IC50 values from the literature [24,25,26,27]. Here, TBX1 is linked to chordoma.