TBX1 and chordoma: To determine whether CuB regulates brachyury expression at the transcriptional or post-transcriptional level, microarray gene expression analyses were performed on ten chordoma cell lines (U-CH1, U-CH2, U-CH11, U-CH14, U-CH17PII, U-CH19, U-CHCF365, MUG-CC1, MUG-Chor1, and UM-Chor1) treated with CuB (50 μM, 48 h) or DMSO as a vehicle control (Figure 6a).