SLC2A1 and hepatocellular carcinoma: The decreased luciferase activity detected following the co-transfection of WT, but not mutant (Figure S4A), GLUT1 3′UTR vector with miR-22 mimic oligonucleotides in the HCC cells demonstrated that miR-22 binds to its complementary binding site in the WT vector preventing the transcription and/or translation of the reporter gene (Figure 4B), proving that GLUT1 is a direct target gene of miR-22.