On the other hand, in the non-capacitation condition, the percentage of spermatozoa with HCC was greater with both concentrations of RAII compared to the UC (p < 0.05), which demonstrates that the activation of Rho GTPase also increases the calcium content in spermatozoa in the absence of capacitation stimuli like albumin and progesterone. This evidence concerns the gene ALB and hepatocellular carcinoma.