For example, a recombinant myxoma virus encoding the soluble wild-type hPD-1 ectodomain induced antitumor CD8+ T-cell responses and tumor eradication in ≈60% of C57BL/6 mice (12/22) bearing subcutaneous B16-F10 melanoma, whereas no animal treated with a control virus displayed a complete response [59]. This evidence concerns the gene PDCD1 and melanoma.