Quantitative PCR (qPCR) confirmed the expression of the human PRKCG transgene; however, total PKCγ protein levels in the C1 domain-mutated PKCγ-G118D line were notably lower, at approximately 52%, compared to a separate C3 domain-mutated transgenic line that manifested severe ataxia and Purkinje cell abnormalities [18]. This evidence concerns the gene PRKCG and cerebellar ataxia.