TNF and infection: The results showed that 2308WT infection promoted the transcription of IL-6, IL-8, and TNF-α, and compared with 2308WT infection, 2308ΔbspF infection increased the transcription of IL-6, IL-8, and TNF-α, indicating that BspF could inhibit the expression of pro-inflammatory factors in cells during Brucella infection (Figure 2A).