As reported in Figure 3 and in Table 1, we found that in the samples analyzed, the CDH1 mRNA levels could efficiently discriminate between healthy and primary tumor samples with 100% sensitivity and 90% specificity, with a cut-off range higher that 3.085 a.u. Also, the CDH1/ZEB1 and ratio significantly differentiated healthy cells from primary tumor-derived cells with good sensitivity and specificity (Table 1). Here, ZEB1 is linked to neoplasm.