Less frequent cancer actionable variants were seen in RET (n = 6, 0.15%), TSC2 (n = 6, 0.15%), PTEN (n = 4, 0.10%), VHL (n = 3, 0.08%), BMPR1A (n = 2, 0.05%), MUTYH (n = 2, 0.05%), RB1 (n = 2, 0.05%), APC (n = 1, 0.03%), MEN1 (n = 1, 0.03%), NF2 (n = 1, 0.03%), SMAD4 (n = 1, 0.03%), STK11 (n = 1, 0.03%), and TP53 (n = 1, 0.03%) genes. Here, SMAD4 is linked to cancer.