Changes in cell morphology, reduced cell proliferation and size, DNA synthesis inhibition, Akt downregulation, PTEN expression upregulation (inhibitor of the phosphatidylinositol 3-kinase/Akt signaling pathway; poorly differentiated NB shows reduced PTEN protein concentration), and anchorage-independent growth suppression was observed in GRP receptor knockdown BE (2)-C NB cells [34]. This evidence concerns the gene AKT1 and neuroblastoma.