Although preventive and control measures have been implemented, containment of bovine respiratory diseases continues to present a formidable challenge, highlighting the need for innovative diagnostic and intervention strategies.<h4>Methods</h4>In this study, we designed specific primers targeting six conserved pathogen genes (<i>N</i> of BRSV, <i>M</i> of BPIV3, <i>5'UTR</i> of BVDV, <i>Hexon</i> of BAV3, <i>oppF</i> of Mb, and <i>gB</i> of IBRV). Here, MB is linked to respiratory system disorder.