PUFAs from ovarian cancer ascites enhance M2-like TAM polarization by inhibiting RhoA-YAP1 signaling, leading to tumor progression. The restoration of YAP1 expression through MST1/2 inhibition with XMU MP1 promotes M1-like polarization, increases CD8+ T cell infiltration, and reduces metastasis. Here, MST1 is linked to ovarian cancer.