Mechanistically, MCT4 alleviated hepatic steatosis by regulating a group of hub genes such as <i>Arg2</i>, <i>Olr1</i>, <i>Cd74</i>, <i>Mmp8, Irf7</i>, <i>Spp1</i>, and <i>Apoe</i>, which in turn impacted multiple pathways involved in lipid metabolism and inflammatory response, such as PPAR, HIF-1, TNF, IL-17, PI3K-AKT, Wnt, and JAK-STAT. This evidence concerns the gene APOE and fatty liver disease.