The three core genes of FN1, TNF, and IL-6 were confirmed using qRT-PCR and Western blotting, and the results were consistent with the transcriptome sequencing results.<h4>Conclusion</h4>Knocking out APE1 can affect the function, related pathways, and downstream target gene expression of ESCC cells. The gene discussed is IL6; the disease is esophageal squamous cell carcinoma.