Since we aimed to investigate the tumor immune microenvironment, we needed to use C57BL/6 mice, which have normal immune systems, so we used the mouse LUAD cell line Lewis Lung Carcinoma (LLC) to establish a subcutaneous tumor model; therefore, the sequence of Flt3L in the recombinant adenoviral vector was a murine sequence. Here, FLT3LG is linked to neoplasm.