OSW-1-induced necroptosis was abrogated by inhibition or knockdown (KD) of MLKL, but not RIP1 or RIP3, in CRC cell lines with or without RIP3 expression, including RIP3- HCT116, RKO, and Lim2405 cells, and RIP3+ LoVo and Lim1215 cells (Fig. 1D–G and Supplementary Fig. 2B–G). This evidence concerns the gene MLKL and colorectal carcinoma.