The lower cancer cell count in the LN2A samples may in part be explained by technical biases in scRNA-Seq, such as loss of sample due to necrosis of tumor cells, or the immune-rich environment of lymph node metastases, leading to increased infiltration of smaller sized immune cells, as illustrated by CD45 and pan-cytokeratin staining (Supplemental Figure 12). The gene discussed is PTPRC; the disease is neoplasm.