To evaluate the effects of 2 of the most common LUSC tumor suppressors — TRP53 and PTEN (Supplemental Figure 1; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.171364DS1) — in a Sox2hi model of NSCLC (11), we intratracheally instilled sgRNAs in a LentiCRISPRv2Cre vector into SNL mice (11, 20) (Figure 1A). Here, TP53 is linked to neoplasm.