TSC1 and neoplasm: As expected, we detected positive enrichment of gRNAs targeting tumor suppressors, PTEN, TSC1, and TSC2, in both DMSO- and imlunestrant-treated cells on days 14 and 31, indicating that loss of PTEN and TSC1/2 enhance tumor cell growth without and with imlunestrant treatment (Figure 6, B–E).