This work shows the effects of a cocktail of cytokines (Il‐1α, TNF‐α, and C1q) and the AD biomarkers Aβ1‐42 and tau on the human astrocytic U‐251 cell line with respect to endogenous Ca2+‐ATPase activity and expression levels of specific PMCA isoforms. This evidence concerns the gene TNF and Alzheimer disease.