<h4>Context</h4>The association between serum soluble Klotho (sKlotho) and chronic kidney disease (CKD) in individuals with diabetes mellitus (DM) remains controversial, and the influence of hypertension on this association is inconclusive.<h4>Objective</h4>This study aims to investigate the joint association of sKlotho and hypertension with CKD prevalence in adults with DM.<h4>Methods</h4>This cross-sectional study included 3,302 adults with DM from the National Health and Nutrition Examination Survey (2007-2016). This evidence concerns the gene KL and Hypertension.