The result emphasized the potential engagement of splicing disorders driven by increased ESRP1 expression in breast cancer cell transformation.[60, 61, 62] Finally, we applied MRAS onto a single cell RNA seq data to investigate the splicing dysregulation during the neuron development process, which has been previously reported to be regulated by splicing in a tightly controlled manner[63] (Figure 5L). This evidence concerns the gene ESRP1 and breast cancer.