Finally, combination therapy with PD-L1 blockade markedly suppressed tumor growth in MB49-bearing mice, which exhibit resistance to PD-L1 blockade monotherapy.<h4>Conclusions</h4>The present study successfully designed and developed Man-MSA-mIFNα, which efficiently induces the CD169<sup>+</sup> phenotype in LN macrophages, contributing to the antitumor immunity. Here, SIGLEC1 is linked to neoplasm.