SIGLEC1 and cancer: CD169-diphtheria toxin (DT) receptor (CD169-DTR) mice in which LN CD169<sup>+</sup> macrophages can be depleted by DT injection were used to verify whether the antitumor effect of Man-MSA-mIFNα is dependent on LN CD169<sup>+</sup> macrophages.<h4>Results</h4>Multiplex tissue imaging predicted close proximity of CD169<sup>+</sup> macrophages and T cells and positive correlation between the number of CD169<sup>+</sup> macrophages and T cells in neighborhoods in LNs of cancer patients.