To validate whether the enhanced neural excitability in iGlut carrying the AD protective allele of rs1532278 was due to the increased expression of CLU, we used CRISPR/Cas9 to introduce a ~ 200 bp homologous deletion in the OCR flanking rs1532278 without disrupting the flanking exons in both iPSC lines (Fig. 3A,Fig. S4 A). This evidence concerns the gene CLU and Alzheimer disease.