SPIN1 and Alzheimer disease: To validate whether the enhanced neural excitability in iGlut carrying the AD protective allele of rs1532278 was due to the increased expression of CLU, we used CRISPR/Cas9 to introduce a ~ 200 bp homologous deletion in the OCR flanking rs1532278 without disrupting the flanking exons in both iPSC lines (Fig. 3A,Fig. S4 A).