We firstly found that the high expression of TSPYL5 in NB tumors and cells (Supplementary Fig. 1a, b), and the single-cell transcriptomes of NB tissues further demonstrated its specific expression in neuroendocrine (NE) cells (Supplementary Fig. 1c–f), the representative malignant cells of NB tumors [17]. This evidence concerns the gene TSPYL5 and neuroblastoma.