Additionally, the knockdown of TSPYL5 significantly reduced the number of cell colonies and the size of sphere as well as the ratio of side population cells (Fig. 2C–E), clearly inhibited the invasiveness and migration of NB cells (Fig. 2F, G) and while the exogenous expression of TSPYL5 rescued the above phenotypes (Fig. 2C–G). Here, TSPYL5 is linked to neuroblastoma.