Using ChIP-qPCR, we detected higher pS5-Pol II at the TSS and decreased pS2-Pol II at the TES (Supplementary Fig. 4e), which resulted in a higher pS5(TSS)/pS2 (TES) ratio (Fig. 3j) at the MYC gene in CHMP5-KD T-ALL cells. Here, MYC is linked to acute lymphoblastic leukemia.