Unlike cells with a defective ESCRT machinery that show impaired membrane receptor dynamics, increased cell size and DNA content25,30, CHMP5-KD and control T-ALL cells displayed similar DNA content (Supplementary Fig. 1e), cell size (FSC) and expressed comparable levels of CD4, CD8 and TCR, the latter downregulated by anti-CD3 cross-linking antibodies similar to control cells (Supplementary Fig. 1f, g). Here, CHMP5 is linked to acute lymphoblastic leukemia.