To elucidate CHMP5 function in T-ALL pathogenesis, we used short hairpins RNA (shRNA) to “knock-down” (KD) CHMP5 (Supplementary Fig. 1a) in CUTLL1 cells, a CD4+CD8+ human T-ALL caused by a t(7;9) (q34;q34) translocation of NOTCH1 into the TCRB loci that results in constitutive cleavage of NOTCH1 into ICN1 by γ-secretase29. Here, CHMP5 is linked to acute lymphoblastic leukemia.