Remarkably, it was stated that overexpressing secreted SEMA3A and Sema3F significantly decreased colony formation and proliferation in soft agar in human GBM cells U87MG, while the expression of homologous family members SEMA3B, SEMA3D, and SEMA3G did not significantly alter the culture [56]. The gene discussed is SEMA3D; the disease is glioblastoma.