To validate the RNA-Seq data for NB cell lines, we analysed the protein expression of PVR and PVRL2 on the surface of the following cell lines by flow cytometry: LAN-1, LS, SK-N-LO, SH-SY5Y, SK-N-AS, SH-EP and Kelly (Fig. 1c). The gene discussed is NECTIN2; the disease is neuroblastoma.