IL33 and melanoma: In this setting, PKH67 green-labeled B16.F10 melanoma cells embedded in Matrigel are loaded in the two opposite chambers alone or with added IL-33, DAC or the combo DAC/IL-33, and confronted for their capacity to attract PKH26 red-labeled spleen cells from naïve mice, either ST2−/− or WT, loaded into the middle fluidic (Supplementary Fig. 1).