Images (Fig. 6A) and quantitative analysis of red fluorescence in the melanoma chambers (Fig. 6B) show that DAC/IL-33 was the most effective treatment inducing the infiltration of WT spleen cells, revealed by preferential displacement of the immune cells towards the right chamber containing melanoma cells exposed to the combo treatment as opposed to the left chamber containing either single drug-treated (IL-33 or DAC) or untreated tumor cells. This evidence concerns the gene IL33 and neoplasm.