Flow cytometry analysis revealed no significant differences in the number of myeloid cells (CD11b +), neutrophils (Ly6G +), or macrophages (F4/80 +), between IgG control and anti-VISTA treated groups in both primary tumours (Fig. 2b–d, Supplementary Fig. 2A) and metastatic lungs (Fig. 2e–g, Supplementary Fig. 2B). Here, VSIR is linked to neoplasm.