Consistent with an upregulation of VISTA in TNBC tissue compared to adjacent normal tissue, exposure of macrophages to TCM induced a significant upregulation of Vsir (encoding VISTA) (Fig. 3b), and VISTA protein expression on the cell surface (Fig. 3c) on tTEMs, suggesting that cancer cells regulate VISTA expression on macrophages. The gene discussed is VSIR; the disease is cancer.