Since neuroimmune organoids contain microglia and astrocytes, to determine whether neuroimmune organoids can replicate neuroinflammation in AD brains, we measured the mRNA expression of the pro-inflammatory cytokine, IL-6, and chemokine, CCL2, by qRT-PCR in the organoids at four to six weeks post-exposure to AD brain extracts or vehicle treatments. This evidence concerns the gene CCL2 and Alzheimer disease.