Further analysis of Hallmark pathway enrichment revealed that allograft rejection, interferon-γ response, and interferon-α response pathways were enriched in all the tumor clusters, while tumor necrosis factor–α (TNF-α) signaling via NF-κB and epithelial mesenchymal transition pathways were distinctly enriched in different subclusters (Fig. 7H). This evidence concerns the gene NFKB1 and neoplasm.