Tregitopes may be applied anywhere active immune response is activated, including in the treatment of autoimmune disease (41), in the treatment of inflammatory conditions (42), in the suppression of transplant rejection (37), and to induce tolerance to therapeutic proteins including monoclonal antibodies, “replacement” proteins such as FVIII (35), and alglucosidase alfa (Myozyme) (43). Here, F8 is linked to autoimmune disease.