To further verify the role of TGFB1 and CXCL5 in LCN2-related CRC migration and invasion, DLD-1-LV-shLCN2 and DLD-1-LV-shNC cells were administrated with TGF-βR1 inhibitor (SB431542) and CXCR2 antagonist (SB225002), respectively. The gene discussed is CXCL5; the disease is colorectal carcinoma.