To unveil the role of LCN2 in CRC metastasis, HCT-8 cells with relatively low LCN2 expression and DLD-1 cells with relatively high LCN2 expression were selected to construct HCT-8-LV-LCN2 and DLD-1- LV-shLCN2 stable cell lines using recombinant lentivirus transfection (Figure 2B). The gene discussed is LCN2; the disease is colorectal carcinoma.