To understand how combination ICI impairs cognitive function and contributes to neuronal damage in our melanoma model, we investigated the expression of neuronal markers that are indicators of neuronal health, including pre- and post-synaptic density proteins (synaptophysin and PSD-95, respectively, Fig. 2B–I) and myelination using myelin basic protein (MBP, Fig. 2J–K). This evidence concerns the gene DLG4 and melanoma.