Therefore, we investigated the role of SPTBN1-cleaved fragments in ammonia-mediated alterations in TGF-β–SMAD3 signaling, utilizing siRNA targeting SPTBN1 (siSPTBN1) in SW480 and Caco-2 human colon cancer cells and treated cells with ammonia. The gene discussed is SMAD3; the disease is malignant colon neoplasm.