This hypothesis was confirmed using EGFR FISH, which revealed EGFR amplification (based on the definition of Colorado Scoring Criteria) in all cases with a low IC Ct value, including three with large EGFR gene clusters and one with at least 15 copies of the EGFR signals in ≥10% of tumor cells (Figure 2 and Figure 3). This evidence concerns the gene EGFR and neoplasm.