To assess the potential mechanism of action between AFC and aerobic glycolysis in colon cancer cells, we used DOCK docking software to predict the binding activities of six active components of AFC (oleanolic acid, eugenol, chlorogenic acid, rhamnetin, kaempferol, and quercetin) with the metabolic target PKM2, and we also investigated the binding activities of the components with the typical high-expression targets of colon cancer, c-myc and cyclin D1. The gene discussed is PKM; the disease is malignant colon neoplasm.