In our study, as expected, mice experienced LPS caused depression-like behaviors in all of these tests, mainly displayed decreased sucrose preference rate in the SPT, reduced total distance moved in the OFT experiment, and increased immobility time in the FST and TST, which were ameliorated by subsequent KF1 treatment, indicating that KF1 was effective in alleviating depression of LPS-induced mice. Here, RNF103 is linked to depressive symptom measurement.